Synthesis of ribonucleic acid by isolated rat liver mitochondria
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چکیده
منابع مشابه
Ribonucleic acid synthesis in isolated mitochondria from Tetrahymena.
Some conditions of RNA synthesis in vitro in isolated mitochondria of Tetrahymena pyriformis were investigated. Sucrose gradient centrifugation analyses and methylated albumin-coated Kieselguhr column chromatography of the RNA synthesized in vitro demonstrate that RNA ranging from 14 S to 18 S, not transfer RNA, was synthesized. Furthermore, the RNA is hybridizable with mitochondrial DNA, and t...
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Isolated rat liver mitochondria can incorporate tritiated thymidine into DNA. The addition of deoxyribonucleotides and ATP are necessary. The process of incorporation was found to be inhibited to the extent of 92% by actinomycin D and only 14% by pancreatic deoxyribonuclease. The identification of the labeled product as mitochondrial DNA is supported by its insolubility in acid, resistance to a...
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The regulation of the fate of acetyl units derived from the P-oxidation of L( -)-palmitylcarnitine was studied using isolated rat liver mitochondria. Citrate synthesis from acetyl-CoA was dependent upon the concentration of Lmalate, the immediate precursor of oxalacetate in this system. The independence of the rate of citrate formation on the means used to stimulate respiration, e.g. ADP for co...
متن کاملRNA synthesis in mitochondria isolated from rat liver.
Mitochondrial RNA (mtRNA) was synthesized from purine and pyrimidine nucleosides in coupling with oxidative phosphorylation using isolated mitochondria. The in vivo synthesized mtRNA was adenine-uracil rich and sedimented at about 20 S by sucrose density gradient centrifugation. A major part of the newly synthesized mtRNA was shown to be poly (A)-containing RNA by the resistance to the digestio...
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We have developed a highly efficient DNA-synthesizing system with isolated intact rat liver mitochondria. The ATP requirements for this in organello DNA synthesis are provided by endogenous synthesis in the presence of exogenous ADP and an oxidizable substrate. In this system, mitochondrial DNA synthesis strikingly proceeds at a constant rate for about 5 h at 37 degrees C. Gel electrophoresis, ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1972
ISSN: 0306-3283
DOI: 10.1042/bj1280299